Analysis of replicating mitochondrial DNA by two-dimensional agarose gel electrophoresis.

TitleAnalysis of replicating mitochondrial DNA by two-dimensional agarose gel electrophoresis.
Publication TypeJournal Article
Year of Publication2007
AuthorsReyes, A, Yasukawa, T, Holt, IJ
JournalMethods Mol Biol
Volume372
Pagination219-32
Date Published2007
ISSN1064-3745
KeywordsAnimals, Blotting, Southern, DNA Replication, DNA Restriction Enzymes, DNA, Mitochondrial, Electrophoresis, Gel, Two-Dimensional, Mice, Nucleic Acid Hybridization
Abstract

Replication intermediates can be separated on agarose gels in two dimensions to reveal a wealth of data on mechanisms of DNA replication. When applied to mitochondrial DNA of higher vertebrates, this technique unearthed a host of unexpected findings, the full implications of which are still being absorbed. Here, we describe the procedures we use to isolate intact mitochondrial replication intermediates from liver of higher vertebrates and the process of separating DNA fragments on neutral two-dimensional agarose gels.

DOI10.1007/978-1-59745-365-3_16
Alternate JournalMethods Mol. Biol.
Citation Key10.1007/978-1-59745-365-3_16
PubMed ID18314729
Grant ListMC_U105663140 / / Medical Research Council / United Kingdom