Adduction of human p53 gene by fecal water: an in vitro biomarker of mutagenesis in the human large bowel.

TitleAdduction of human p53 gene by fecal water: an in vitro biomarker of mutagenesis in the human large bowel.
Publication TypeJournal Article
Year of Publication2007
AuthorsGreetham, HL, Bingham, SA, Burns, PA
JournalCancer Epidemiol Biomarkers Prev
Volume16
Issue12
Pagination2681-5
Date Published2007 Dec
ISSN1055-9965
KeywordsBiomarkers, Body Water, DNA Adducts, DNA-Directed DNA Polymerase, Feces, Genes, p53, Humans, Intestine, Large, Mutagenesis, Mutagens, Polymerase Chain Reaction
Abstract

A polymerase arrest assay was designed to determine sites of adduction in the human p53 gene induced by incubation with fecal water. Significant formation of adducts was observed on p53 DNA after a 2-h incubation in fecal water from 10 of 17 samples studied. Large sample-to-sample variation was observed. The major sites of polymerase termination occurred at nucleotides 3' to guanine residues. Adduct sites coincided with colorectal cancer p53 mutation "hotspots," highlighting the potential carcinogenicity of fecal material.

DOI10.1158/1055-9965.EPI-07-0642
Alternate JournalCancer Epidemiol. Biomarkers Prev.
Citation Key10.1158/1055-9965.EPI-07-0642
PubMed ID18086774
Grant ListMC_U105630924 / / Medical Research Council / United Kingdom