Mechanism of inhibition of bovine F1-ATPase by resveratrol and related polyphenols.

TitleMechanism of inhibition of bovine F1-ATPase by resveratrol and related polyphenols.
Publication TypeJournal Article
Year of Publication2007
AuthorsGledhill, JR, Montgomery, MG, Leslie, AGW, Walker, JE
JournalProc Natl Acad Sci U S A
Volume104
Issue34
Pagination13632-7
Date Published2007 Aug 21
ISSN0027-8424
KeywordsAnimals, Binding Sites, Cattle, Crystallography, X-Ray, Enzyme Inhibitors, Flavonoids, Models, Molecular, Phenols, Polyphenols, Protein Binding, Protein Structure, Quaternary, Protein Structure, Tertiary, Protein Subunits, Proton-Translocating ATPases, Resveratrol, Stilbenes
Abstract

The structures of F(1)-ATPase from bovine heart mitochondria inhibited with the dietary phytopolyphenol, resveratrol, and with the related polyphenols quercetin and piceatannol have been determined at 2.3-, 2.4- and 2.7-A resolution, respectively. The inhibitors bind to a common site in the inside surface of an annulus made from loops in the three alpha- and three beta-subunits beneath the "crown" of beta-strands in their N-terminal domains. This region of F(1)-ATPase forms a bearing to allow the rotation of the tip of the gamma-subunit inside the annulus during catalysis. The binding site is a hydrophobic pocket between the C-terminal tip of the gamma-subunit and the beta(TP) subunit, and the inhibitors are bound via H-bonds mostly to their hydroxyl moieties mediated by bound water molecules and by hydrophobic interactions. There are no equivalent sites between the gamma-subunit and either the beta(DP) or the beta(E) subunit. The inhibitors probably prevent both the synthetic and hydrolytic activities of the enzyme by blocking both senses of rotation of the gamma-subunit. The beneficial effects of dietary resveratrol may derive in part by preventing mitochondrial ATP synthesis in tumor cells, thereby inducing apoptosis.

DOI10.1073/pnas.0706290104
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
Citation Key10.1073/pnas.0706290104
PubMed ID17698806
PubMed Central IDPMC1948022
Grant ListMC_U105184325 / / Medical Research Council / United Kingdom
MC_U105663150 / / Medical Research Council / United Kingdom