Reproducible improvements in order and diffraction limit of crystals of bovine mitochondrial F(1)-ATPase by controlled dehydration.

TitleReproducible improvements in order and diffraction limit of crystals of bovine mitochondrial F(1)-ATPase by controlled dehydration.
Publication TypeJournal Article
Year of Publication2006
AuthorsBowler, MW, Montgomery, MG, Leslie, AGW, Walker, JE
JournalActa Crystallogr D Biol Crystallogr
Volume62
IssuePt 9
Pagination991-5
Date Published2006 Sep
ISSN0907-4449
KeywordsAdenosine Triphosphatases, Animals, Cattle, Crystallization, Crystallography, X-Ray, Dehydration, Mitochondria, Models, Molecular, Models, Statistical, Proton-Translocating ATPases, Reproducibility of Results, Submitochondrial Particles, Time Factors
Abstract

Orthorhombic crystals of bovine F(1)-ATPase have been subjected to controlled dehydration. A decrease in the relative humidity surrounding the crystals to 90% reproducibly reduced their unit-cell volume by 22% (950,000 Angstrom(3)) and improved the diffraction limit and mosaic spread of the crystals significantly. These dehydrated crystals diffracted X-rays to 1.8 Angstrom resolution at a synchrotron source, the best diffraction limit yet attained with these crystals, although radiation damage limited the resolution of a complete data set to 1.95 Angstrom.

DOI10.1107/S0907444906020877
Alternate JournalActa Crystallogr. D Biol. Crystallogr.
Citation Key10.1107/S0907444906020877
PubMed ID16929099
Grant ListMC_U105184325 / / Medical Research Council / United Kingdom
MC_U105663150 / / Medical Research Council / United Kingdom