Investigating mitochondrial radical production using targeted probes.

TitleInvestigating mitochondrial radical production using targeted probes.
Publication TypeJournal Article
Year of Publication2004
AuthorsMurphy, MP
JournalBiochem Soc Trans
Volume32
IssuePt 6
Pagination1011-4
Date Published2004 Dec
ISSN0300-5127
KeywordsAntioxidants, Free Radicals, Intracellular Membranes, Lipid Bilayers, Membrane Potentials, Mitochondria, Models, Biological, Oxidative Stress, Oxygen Consumption, Reactive Oxygen Species, Ubiquinone, Vitamin D
Abstract

A range of mitochondria-targeted probe molecules that comprise a lipophilic cation covalently attached to an active moiety have been developed. The lipophilic cation causes the accumulation of these molecules into mitochondria, driven by the mitochondrial membrane potential. To date, we have targeted antioxidants, spin traps, thiol reagents and DNA-alkylating compounds to mitochondria. The selective mitochondrial localization of these compounds enables us to investigate several aspects of the production of reactive oxygen species by mitochondria.

DOI10.1042/BST0321011
Alternate JournalBiochem. Soc. Trans.
Citation Key10.1042/BST0321011
PubMed ID15506950