Conjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cells.

TitleConjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cells.
Publication TypeJournal Article
Year of Publication2004
AuthorsMuratovska, A, Eccles, MR
JournalFEBS Lett
Volume558
Issue1-3
Pagination63-8
Date Published2004 Jan 30
ISSN0014-5793
KeywordsAnimals, Carrier Proteins, Cell Membrane, Cell-Penetrating Peptides, Cercopithecus aethiops, CHO Cells, COS Cells, Cricetinae, Cricetulus, Cytoplasm, Disulfides, Fibroblasts, Galanin, Gene Silencing, Gene Transfer Techniques, Genes, Reporter, Green Fluorescent Proteins, Luciferases, Luminescent Proteins, Mice, Peptide Fragments, Recombinant Fusion Proteins, RNA, Small Interfering, Transgenes, Wasp Venoms
Abstract

The efficient delivery of short interfering RNAs (siRNAs) into cells provides a powerful approach to study cellular functions. SiRNAs were coupled to the membrane permeant peptides (MPPs) penetratin and transportan to improve their uptake by cells. Thiol-containing siRNAs corresponding to luciferase, or green fluorescent protein (GFP) transgenes, were synthesized and conjugated to penetratin or transportan via a disulfide bond that is labile in the reducing environment of the cytoplasm. These MPP-siRNAs efficiently reduced transient and stable expression of reporter transgenes in several mammalian cell types in a high proportion of cells, and demonstrated equivalent or better delivery characteristics than cationic liposomes with fewer manipulations.

DOI10.1016/S0014-5793(03)01505-9
Alternate JournalFEBS Lett.
Citation Key10.1016/S0014-5793(03)01505-9
PubMed ID14759517