Superoxide activates a GDP-sensitive proton conductance in skeletal muscle mitochondria from king penguin (Aptenodytes patagonicus).

TitleSuperoxide activates a GDP-sensitive proton conductance in skeletal muscle mitochondria from king penguin (Aptenodytes patagonicus).
Publication TypeJournal Article
Year of Publication2003
AuthorsTalbot, DA, Hanuise, N, Rey, B, Rouanet, J-L, Duchamp, C, Brand, MD
JournalBiochem Biophys Res Commun
Volume312
Issue4
Pagination983-8
Date Published2003 Dec 26
ISSN0006-291X
KeywordsAnimals, Base Sequence, Birds, Carrier Proteins, Cell Membrane, Cell Respiration, Cells, Cultured, Chickens, Electric Conductivity, Guanosine Diphosphate, Ion Channels, Membrane Potentials, Membrane Proteins, Mitochondria, Muscle, Mitochondrial Proteins, Molecular Sequence Data, Muscle, Skeletal, Oxygen, Proton Pumps, Protons, Sequence Alignment, Sequence Homology, Nucleic Acid, Species Specificity, Superoxides, Turkeys, Uncoupling Protein 1
Abstract

We present the partial nucleotide sequence of the avian uncoupling protein (avUCP) gene from king penguin (Aptenodytes patagonicus), showing that the protein is 88-92% identical to chicken (Gallus gallus), turkey (Meleagris gallopavo), and hummingbird (Eupetomena macroura). We show that superoxide activates the proton conductance of mitochondria isolated from king penguin skeletal muscle. GDP abolishes the superoxide-activated proton conductance, indicating that it is mediated via avUCP. In the absence of superoxide there is no GDP-sensitive component of the proton conductance from penguin muscle mitochondria demonstrating that avUCP plays no role in the basal proton leak.

DOI10.1016/j.bbrc.2003.11.022
Alternate JournalBiochem. Biophys. Res. Commun.
Citation Key10.1016/j.bbrc.2003.11.022
PubMed ID14651968