Mammalian mitochondrial DNA replicates bidirectionally from an initiation zone.

TitleMammalian mitochondrial DNA replicates bidirectionally from an initiation zone.
Publication TypeJournal Article
Year of Publication2003
AuthorsBowmaker, M, Yang, MYao, Yasukawa, T, Reyes, A, Jacobs, HT, Huberman, JA, Holt, IJ
JournalJ Biol Chem
Volume278
Issue51
Pagination50961-9
Date Published2003 Dec 19
ISSN0021-9258
KeywordsAnimals, Cytochromes b, DNA Replication, DNA, Mitochondrial, Electrophoresis, Gel, Two-Dimensional, Genome, Humans, Mice, Models, Genetic, NADH Dehydrogenase, Rats, Replication Origin
Abstract

Previous data from our laboratory suggested that replication of mammalian mitochondrial DNA initiates exclusively at or near to the formerly designated origin of heavy strand replication, OH, and proceeds unidirectionally from that locus. New results obtained using two-dimensional agarose gel electrophoresis of replication intermediates demonstrate that replication of mitochondrial DNA initiates from multiple origins across a broad zone. After fork arrest near OH, replication is restricted to one direction only. The initiation zone of bidirectional replication includes the genes for cytochrome b and NADH dehydrogenase subunits 5 and 6.

DOI10.1074/jbc.M308028200
Alternate JournalJ. Biol. Chem.
Citation Key10.1074/jbc.M308028200
PubMed ID14506235
Grant ListMC_U105663140 / / Medical Research Council / United Kingdom
CA84302 / CA / NCI NIH HHS / United States
CA95908 / CA / NCI NIH HHS / United States
GM49294 / GM / NIGMS NIH HHS / United States