Quantification of isoflavones and lignans in serum using isotope dilution liquid chromatography/tandem mass spectrometry.

TitleQuantification of isoflavones and lignans in serum using isotope dilution liquid chromatography/tandem mass spectrometry.
Publication TypeJournal Article
Year of Publication2003
AuthorsGrace, PB, Taylor, JI, Botting, NP, Fryatt, T, Oldfield, MF, Al-Maharik, N, Bingham, SA
JournalRapid Commun Mass Spectrom
Volume17
Issue12
Pagination1350-7
Date Published2003
ISSN0951-4198
KeywordsCalibration, Chromatography, Liquid, Estrogens, Non-Steroidal, Female, Humans, Isoflavones, Isotopes, Lignans, Male, Mass Spectrometry, Phytoestrogens, Plant Preparations, Reproducibility of Results, Sensitivity and Specificity, United Kingdom
Abstract

Phytoestrogens (isoflavones and lignans) are receiving increasing attention due to a potential protective effect against a number of complex diseases. However, in order to investigate these associations, it is necessary to accurately quantify the levels of phytoestrogens in foods and biological fluids. We report an assay for three isoflavones (daidzein, genistein, and glycitein), two metabolites of daidzein (O-desmethylangolensin and equol), and two lignans (enterodiol and enterolactone) in human serum using electrospray ionisation liquid chromatography/mass spectrometry (LC/MS) with selective reaction monitoring. A simple, highly automated sample preparation procedure requires only 200 microL of sample and utilises one solid-phase extraction stage. Limits of detection are in the region of 10 pg/mL for all analytes except equol, which had a limit of detection of approximately 100 pg/mL. The method developed is suitable for measuring the concentrations of phytoestrogens in blood samples collected from large epidemiological studies. The results of the analysis of serum samples from 300 men and women living in the UK are reported.

DOI10.1002/rcm.1059
Alternate JournalRapid Commun. Mass Spectrom.
Citation Key10.1002/rcm.1059
PubMed ID12811759