|Title||Characterisation of new intracellular membranes in Escherichia coli accompanying large scale over-production of the b subunit of F(1)F(o) ATP synthase.|
|Publication Type||Journal Article|
|Year of Publication||2000|
|Authors||Arechaga, I, Miroux, B, Karrasch, S, Huijbregts, R, de Kruijff, B, Runswick, MJ, Walker, JE|
|Date Published||2000 Oct 06|
|Keywords||Escherichia coli, Intracellular Membranes, Lipids, Peptide Fragments, Phospholipids, Protein Conformation, Proton-Translocating ATPases|
Recombinant membrane proteins in Escherichia coli are either expressed at relatively low level in the cytoplasmic membrane or they accumulate as inclusion bodies. Here, we report that the abundant over-production of subunit b of E. coli F(1)F(o) ATP synthase in the mutant host strains E. coli C41(DE3) and C43(DE3) is accompanied by the proliferation of intracellular membranes without formation of inclusion bodies. Maximal levels of proliferation of intracellular membranes were observed in C43(DE3) cells over-producing subunit b. The new proliferated membranes contained all the over-expressed protein and could be recovered by a single centrifugation step. Recombinant subunit b represented up to 80% of the protein content of the membranes. The lipid:protein ratios and phospholipid compositions of the intracellular membranes differ from those of bacterial cytoplasmic membranes, and they are particularly rich in cardiolipin.
|Alternate Journal||FEBS Lett.|