Detection and quantification of sucrose as dietary biomarker using gas chromatography and liquid chromatography with mass spectrometry.

TitleDetection and quantification of sucrose as dietary biomarker using gas chromatography and liquid chromatography with mass spectrometry.
Publication TypeJournal Article
Year of Publication2008
AuthorsKuhnle, GGC, Joosen, AMCP, Wood, TR, Runswick, SA, Griffin, JL, Bingham, SA
JournalRapid Commun Mass Spectrom
Volume22
Issue3
Pagination279-82
Date Published2008
ISSN0951-4198
KeywordsBiomarkers, Chromatography, High Pressure Liquid, Dietary Carbohydrates, Gas Chromatography-Mass Spectrometry, Humans, Reproducibility of Results, Sensitivity and Specificity, Sucrose, Urinalysis
Abstract

Several epidemiological studies suggest a link between the intake of refined sugars and an increased risk for colorectal, breast, pancreatic and endometrial cancer. However, other studies failed to confirm these conclusions and the reason for this may be the ambiguity of dietary assessment methods - mainly self-reporting - employed. Sucrose is an established biomarker for sugars intake, allowing the objective assessment of dietary sucrose. So far, urinary excretion of sucrose was mainly determined using an enzyme assay. However, this method is time-consuming and labour-intensive. In this study, we present a mass spectrometric method for the determination of sucrose in urine using liquid chromatography with mass spectrometry (LC/MS) which can be used for large-scale epidemiological studies.

DOI10.1002/rcm.3355
Alternate JournalRapid Commun. Mass Spectrom.
Citation Key10.1002/rcm.3355
PubMed ID18181249
Grant ListMC_U105630924 / / Medical Research Council / United Kingdom