Were inefficient mitochondrial haplogroups selected during migrations of modern humans? A test using modular kinetic analysis of coupling in mitochondria from cybrid cell lines.

TitleWere inefficient mitochondrial haplogroups selected during migrations of modern humans? A test using modular kinetic analysis of coupling in mitochondria from cybrid cell lines.
Publication TypeJournal Article
Year of Publication2007
AuthorsAmo, T, Brand, MD
JournalBiochem J
Volume404
Issue2
Pagination345-51
Date Published2007 Jun 01
ISSN1470-8728
KeywordsAnimals, Arctic Regions, Biological Evolution, Catalase, Cell Line, Tumor, DNA, Mitochondrial, Haplotypes, Humans, Hybrid Cells, Kinetics, Membrane Potentials, Mice, Oxidative Phosphorylation, Travel
Abstract

We introduce a general test of the bioenergetic importance of mtDNA (mitochondrial DNA) variants: modular kinetic analysis of oxidative phosphorylation in mitochondria from cybrid cells with constant nuclear DNA but different mtDNA. We have applied this test to the hypothesis [Ruiz-Pesini, Mishmar, Brandon, Procaccio and Wallace (2004) Science 303, 223-226] that particular mtDNA haplogroups (specific combinations of polymorphisms) that cause lowered coupling efficiency, leading to generation of less ATP and more heat, were positively selected during radiations of modern humans into colder climates. Contrary to the predictions of this hypothesis, mitochondria from Arctic haplogroups had similar or even greater coupling efficiency than mitochondria from tropical haplogroups.

DOI10.1042/BJ20061609
Alternate JournalBiochem. J.
Citation Key10.1042/BJ20061609
PubMed ID17355224
PubMed Central IDPMC1868799
Grant ListMC_U105663137 / / Medical Research Council / United Kingdom