Replication of vertebrate mitochondrial DNA entails transient ribonucleotide incorporation throughout the lagging strand.

TitleReplication of vertebrate mitochondrial DNA entails transient ribonucleotide incorporation throughout the lagging strand.
Publication TypeJournal Article
Year of Publication2006
AuthorsYasukawa, T, Reyes, A, Cluett, TJ, Yang, M-Y, Bowmaker, M, Jacobs, HT, Holt, IJ
JournalEMBO J
Volume25
Issue22
Pagination5358-71
Date Published2006 Nov 15
ISSN0261-4189
Keywords5' Flanking Region, Animals, Chickens, DNA Replication, DNA, Mitochondrial, Electrophoresis, Agar Gel, Electrophoresis, Gel, Two-Dimensional, Mice, Mitochondria, Liver, Nucleic Acid Conformation, Rats, Ribonucleotides, RNA
Abstract

Using two-dimensional agarose gel electrophoresis, we show that mitochondrial DNA (mtDNA) replication of birds and mammals frequently entails ribonucleotide incorporation throughout the lagging strand (RITOLS). Based on a combination of two-dimensional agarose gel electrophoretic analysis and mapping of 5' ends of DNA, initiation of RITOLS replication occurs in the major non-coding region of vertebrate mtDNA and is effectively unidirectional. In some cases, conversion of nascent RNA strands to DNA starts at defined loci, the most prominent of which maps, in mammalian mtDNA, in the vicinity of the site known as the light-strand origin.

DOI10.1038/sj.emboj.7601392
Alternate JournalEMBO J.
Citation Key10.1038/sj.emboj.7601392
PubMed ID17066082
PubMed Central IDPMC1636616
Grant ListMC_U105663140 / / Medical Research Council / United Kingdom