Yeast mitochondrial ADP/ATP carriers are monomeric in detergents.

TitleYeast mitochondrial ADP/ATP carriers are monomeric in detergents.
Publication TypeJournal Article
Year of Publication2006
AuthorsBamber, L, Harding, M, P Butler, JG, Kunji, ERS
JournalProc Natl Acad Sci U S A
Volume103
Issue44
Pagination16224-9
Date Published2006 Oct 31
ISSN0027-8424
KeywordsAnimals, Cattle, Chromatography, Gel, Detergents, Micelles, Mitochondrial ADP, ATP Translocases, Molecular Weight, Saccharomyces cerevisiae
Abstract

Mitochondrial carriers are believed widely to be homodimers both in the inner membrane of the organelle and in detergents. The dimensions and molecular masses of the detergent and protein-detergent micelles were measured for yeast ADP/ATP carriers in a range of different detergents. The radius of the carrier at the midpoint of the membrane, its average radius, its Stokes' radius, its molecular mass, and its excluded volume were determined. These parameters are consistent with the known structural model of the bovine ADP/ATP carrier and they demonstrate that the yeast mitochondrial ADP/ATP carriers are monomeric in detergents. Therefore, models of substrate transport have to be considered in which the carrier operates as a monomer rather than as a dimer.

DOI10.1073/pnas.0607640103
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
Citation Key10.1073/pnas.0607640103
PubMed ID17056710
PubMed Central IDPMC1618811