Elucidating the mechanisms of coupled electron transfer and catalytic reactions by protein film voltammetry.

TitleElucidating the mechanisms of coupled electron transfer and catalytic reactions by protein film voltammetry.
Publication TypeJournal Article
Year of Publication2006
AuthorsHirst, J
JournalBiochim Biophys Acta
Volume1757
Issue4
Pagination225-39
Date Published2006 Apr
ISSN0006-3002
KeywordsCatalysis, Electrochemistry, Electron Transport, Proteins, Thermodynamics
Abstract

Protein film voltammetry, the direct electrochemistry of redox enzymes and proteins, provides precise and comprehensive information on complicated reaction mechanisms. By controlling the driving force for a reaction (using the applied potential) and monitoring the reaction in real time (using the current), it allows thermodynamic and kinetic information to be determined simultaneously. Two challenges are inherent to protein film voltammetry: (i) to adsorb the protein or enzyme in a native and active configuration on the electrode surface, and (ii) to understand and interpret voltammetric results on both a qualitative and quantitative level, allowing mechanistic models to be proposed and rigorous experiments to test these models to be devised. This review focuses on the second of these two challenges. It describes how to use protein film voltammetry to derive mechanistic and biochemically relevant information about redox proteins and enzymes, and how to evaluate and interpret voltammetric results. Selected key studies are described in detail, to illustrate their underlying principles, strategies and physical interpretations.

DOI10.1016/j.bbabio.2006.04.002
Alternate JournalBiochim. Biophys. Acta
Citation Key10.1016/j.bbabio.2006.04.002
PubMed ID16730325
Grant ListMC_U105663141 / / Medical Research Council / United Kingdom