Eukaryotic membrane protein overproduction in Lactococcus lactis.

TitleEukaryotic membrane protein overproduction in Lactococcus lactis.
Publication TypeJournal Article
Year of Publication2005
AuthorsKunji, ERS, Chan, KWai, Slotboom, DJan, Floyd, S, O'Connor, R, Monné, M
JournalCurr Opin Biotechnol
Volume16
Issue5
Pagination546-51
Date Published2005 Oct
ISSN0958-1669
KeywordsAnimals, Bacteria, Biotechnology, Escherichia coli, Eukaryotic Cells, Gene Expression, Humans, Lactococcus lactis, Membrane Proteins, Membrane Transport Proteins, Mitochondrial ADP, ATP Translocases, Receptors, Peptide, Recombinant Proteins, Saccharomyces cerevisiae Proteins
Abstract

Eukaryotic membrane proteins play many vital roles in the cell and are important drug targets. Approximately 25% of all genes identified in the genome are known to encode membrane proteins, but the vast majority have no assigned function. Although the generation of structures of soluble proteins has entered the high-throughput stage, for eukaryotic membrane proteins only a dozen high-resolution structures have been obtained so far. One major bottleneck for the functional and structural characterisation of membrane proteins is the overproduction of biologically active material. Recent advances in the development of the Lactococcus lactis expression system have opened the way for the high-throughput functional expression of eukaryotic membrane proteins.

DOI10.1016/j.copbio.2005.08.006
Alternate JournalCurr. Opin. Biotechnol.
Citation Key10.1016/j.copbio.2005.08.006
PubMed ID16143505