Lack of effect of caloric restriction on bioenergetics and reactive oxygen species production in intact rat hepatocytes.

TitleLack of effect of caloric restriction on bioenergetics and reactive oxygen species production in intact rat hepatocytes.
Publication TypeJournal Article
Year of Publication2005
AuthorsLambert, AJ, Merry, BJ
JournalJ Gerontol A Biol Sci Med Sci
Volume60
Issue2
Pagination175-80
Date Published2005 Feb
ISSN1079-5006
KeywordsAdenosine Triphosphate, Animals, Caloric Restriction, Cell Surface Extensions, Energy Metabolism, Hepatocytes, Male, Mitochondria, Oxygen Consumption, Rats, Rats, Inbred BN, Reactive Oxygen Species
Abstract

To investigate the hypothesis that caloric restriction alters mitochondrial function in situ, intact hepatocytes were isolated from fully fed and calorie-restricted (55% of control food intake, 4 months duration) male Brown-Norway rats at 6 months of age, and various parameters were determined. Overall, the production of reactive oxygen species was not affected by caloric restriction, neither were the mitochondrial membrane potential, oxygen consumption driving proton leak, or oxygen consumption driving ATP turnover. It is concluded that while isolated mitochondria from liver tissue of calorie-restricted animals display a reduction in the generation of reactive oxygen species, it was not possible to confirm this effect in isolated hepatocytes. Further work is required to establish what effect, if any, caloric restriction has on the rate of generation of reactive oxygen species in intact cells and tissues and importantly at the whole-animal level.

DOI10.1093/gerona/60.2.175
Alternate JournalJ. Gerontol. A Biol. Sci. Med. Sci.
Citation Key10.1093/gerona/60.2.175
PubMed ID15814858