Bidirectional replication initiates at sites throughout the mitochondrial genome of birds.

TitleBidirectional replication initiates at sites throughout the mitochondrial genome of birds.
Publication TypeJournal Article
Year of Publication2005
AuthorsReyes, A, Yang, MYao, Bowmaker, M, Holt, IJ
JournalJ Biol Chem
Volume280
Issue5
Pagination3242-50
Date Published2005 Feb 04
ISSN0021-9258
KeywordsAnimals, Chickens, Chromosome Mapping, DNA Replication, DNA, Mitochondrial, Genome, Mammals, Mitochondria, Liver, NADH Dehydrogenase, Replication Origin
Abstract

Analysis of mitochondrial replication intermediates of Gallus gallus on fork-direction gels indicates that replication occurs in both directions around circular mitochondrial DNA. This finding was corroborated by a study of chick mitochondrial DNA on standard neutral two-dimensional agarose gels, which yielded archetypal initiation arcs in fragments covering the entire genome. There was, however, considerable variation in initiation arc intensity. The majority of initiation events map to regions flanking the major non-coding region, in particular the NADH dehydrogenase subunit 6 (ND6) gene. Initiation point mapping of the ND6 gene identified prominent free 5' ends of DNA, which are candidate start sites for DNA synthesis. Therefore we propose that the initiation zone of G. gallus mitochondrial DNA encompasses most, if not all, of the genome, with preferred initiation sites in regions flanking the major non-coding region. Comparison with mammals suggests a common mechanism of initiation of mitochondrial DNA replication in higher vertebrates.

DOI10.1074/jbc.M411916200
Alternate JournalJ. Biol. Chem.
Citation Key10.1074/jbc.M411916200
PubMed ID15557283
Grant ListMC_U105663140 / / Medical Research Council / United Kingdom