Formation and characterization of an all-ferrous Rieske cluster and stabilization of the [2Fe-2S]0 core by protonation.

TitleFormation and characterization of an all-ferrous Rieske cluster and stabilization of the [2Fe-2S]0 core by protonation.
Publication TypeJournal Article
Year of Publication2004
AuthorsLeggate, EJ, Bill, E, Essigke, T, G Ullmann, M, Hirst, J
JournalProc Natl Acad Sci U S A
Volume101
Issue30
Pagination10913-8
Date Published2004 Jul 27
ISSN0027-8424
KeywordsAnimals, Cattle, Cloning, Molecular, Cysteine, Electron Transport Complex III, Iron-Sulfur Proteins, Kinetics, Ligands, Solutions, Spectroscopy, Mossbauer, Sulfides
Abstract

The all-ferrous Rieske cluster, [2Fe-2S](0), has been produced in solution and characterized by protein-film voltammetry and UV-visible, EPR, and Mössbauer spectroscopies. The [2Fe-2S](0) cluster, in the overexpressed soluble domain of the Rieske protein from the bovine cytochrome bc(1) complex, is formed at -0.73 V at pH 7. Therefore, at pH 7, the [2Fe-2S](1+/0) couple is 1.0 V below the [2Fe-2S](2+/1+) couple. The two cluster-bound ferrous irons are both high spin (S = 2), and they are coupled antiferromagnetically (-J > or = 30 cm(-1), H =-2JS1.S2) to give a diamagnetic (S = 0) ground state. The ability of the Rieske cluster to exist in three oxidation states (2+, 1+, and 0) without an accompanying coupled reaction, such as a conformational change or protonation, is highly unusual. However, uncoupled reduction to the [2Fe-2S](0) state occurs at pH > 9.8 only, and at high pH the intact cluster persists in solution for <1 min. At pH < 9.8, the all-ferrous cluster is stabilized significantly by protonation. A combination of experimental data and calculations based on density functional theory suggests strongly that the proton binds to one of the cluster mu(2)-sulfides, consistent with observations that reduced [3Fe-4S] clusters are protonated also. The implications for our understanding of coupled reactions at iron-sulfur clusters and of the factors that determine the relative stabilities of their different oxidation states are discussed.

DOI10.1073/pnas.0402711101
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
Citation Key10.1073/pnas.0402711101
PubMed ID15263097
PubMed Central IDPMC503719