Mitochondrial matrix reactive oxygen species production is very sensitive to mild uncoupling.

TitleMitochondrial matrix reactive oxygen species production is very sensitive to mild uncoupling.
Publication TypeJournal Article
Year of Publication2003
AuthorsMiwa, S, Brand, MD
JournalBiochem Soc Trans
Volume31
IssuePt 6
Pagination1300-1
Date Published2003 Dec
ISSN0300-5127
KeywordsAnimals, Drosophila, Membrane Potentials, Mitochondria, Reactive Oxygen Species
Abstract

Mitochondria produce ROS (reactive oxygen species) as a by-product of aerobic respiration. Several studies in mammals and birds suggest that the most physiologically relevant ROS production is from complex I following reverse electron flow, and is highly sensitive to membrane potential. A study of Drosophila mitochondria respiring glycerol 3-phosphate revealed that membrane potential-sensitive ROS production from complex I following reverse electron flow was on the matrix side of the inner membrane. A 10 mV decrease in membrane potential was enough to abolish around 70% of the ROS produced by complex I under these conditions. Another important ROS generator in this model, glycerol-3-phosphate dehydrogenase, produced ROS mostly to the cytosolic side; this ROS production was totally insensitive to a small decrease in membrane potential (10 mV). Thus mild uncoupling may be particularly significant for ROS production from complex I on the matrix side of the mitochondrial inner membrane.

DOI10.1042/bst0311300
Alternate JournalBiochem. Soc. Trans.
Citation Key10.1042/bst0311300
PubMed ID14641047