The mitochondrial dicarboxylate and 2-oxoglutarate carriers do not transport glutathione.

TitleThe mitochondrial dicarboxylate and 2-oxoglutarate carriers do not transport glutathione.
Publication TypeJournal Article
Year of Publication2015
AuthorsBooty, LM, King, MS, Thangaratnarajah, C, Majd, H, James, AM, Kunji, ERS, Murphy, MP
JournalFEBS Lett
Volume589
Issue5
Pagination621-8
Date Published2015 Feb 27
ISSN1873-3468
KeywordsDicarboxylic Acid Transporters, Glutathione, Immunoblotting, Ketoglutaric Acids, Lactococcus lactis, Membrane Transport Proteins, Mitochondria, Polymerase Chain Reaction, Reactive Oxygen Species
Abstract

Glutathione carries out vital protective roles within mitochondria, but is synthesised in the cytosol. Previous studies have suggested that the mitochondrial dicarboxylate and 2-oxoglutarate carriers were responsible for glutathione uptake. We set out to characterise the putative glutathione transport by using fused membrane vesicles of Lactococcus lactis overexpressing the dicarboxylate and 2-oxoglutarate carriers. Although transport of the canonical substrates could be measured readily, an excess of glutathione did not compete for substrate uptake nor could transport of glutathione be measured directly. Thus these mitochondrial carriers do not transport glutathione and the identity of the mitochondrial glutathione transporter remains unknown.

DOI10.1016/j.febslet.2015.01.027
Alternate JournalFEBS Lett.
Citation Key10.1016/j.febslet.2015.01.027
PubMed ID25637873
PubMed Central IDPMC4332691
Grant ListMC_U105663139 / / Medical Research Council / United Kingdom
MC_U105663142 / / Medical Research Council / United Kingdom
/ / Medical Research Council / United Kingdom