The structure of F₁-ATPase from Saccharomyces cerevisiae inhibited by its regulatory protein IF₁.

TitleThe structure of F₁-ATPase from Saccharomyces cerevisiae inhibited by its regulatory protein IF₁.
Publication TypeJournal Article
Year of Publication2013
AuthorsRobinson, GC, Bason, JV, Montgomery, MG, Fearnley, IM, Mueller, DM, Leslie, AGW, Walker, JE
JournalOpen Biol
Volume3
Issue2
Pagination120164
Date Published2013 Feb 13
ISSN2046-2441
KeywordsAdenosine Diphosphate, Animals, Binding Sites, Catalysis, Catalytic Domain, Cattle, Crystallography, X-Ray, Hydrolysis, Protein Binding, Protein Conformation, Proteins, Proton-Translocating ATPases, Saccharomyces cerevisiae
Abstract

The structure of F₁-ATPase from Saccharomyces cerevisiae inhibited by the yeast IF₁ has been determined at 2.5 Å resolution. The inhibitory region of IF₁ from residues 1 to 36 is entrapped between the C-terminal domains of the α(DP)- and β(DP)-subunits in one of the three catalytic interfaces of the enzyme. Although the structure of the inhibited complex is similar to that of the bovine-inhibited complex, there are significant differences between the structures of the inhibitors and their detailed interactions with F₁-ATPase. However, the most significant difference is in the nucleotide occupancy of the catalytic β(E)-subunits. The nucleotide binding site in β(E)-subunit in the yeast complex contains an ADP molecule without an accompanying magnesium ion, whereas it is unoccupied in the bovine complex. Thus, the structure provides further evidence of sequential product release, with the phosphate and the magnesium ion released before the ADP molecule.

DOI10.1098/rsob.120164
Alternate JournalOpen Biol
Citation Key10.1098/rsob.120164
PubMed ID23407639
PubMed Central IDPMC3603450
Grant List / / Medical Research Council / United Kingdom
R01 GM066223 / GM / NIGMS NIH HHS / United States
R01GM66223 / GM / NIGMS NIH HHS / United States
MC_U105184325 / / Medical Research Council / United Kingdom
MC_U105663150 / / Medical Research Council / United Kingdom