Mitochondrial nucleoid interacting proteins support mitochondrial protein synthesis.

TitleMitochondrial nucleoid interacting proteins support mitochondrial protein synthesis.
Publication TypeJournal Article
Year of Publication2012
AuthorsHe, J, Cooper, HM, Reyes, A, Di Re, M, Sembongi, H, Litwin, TR, Gao, J, Neuman, KC, Fearnley, IM, Spinazzola, A, Walker, JE, Holt, IJ
JournalNucleic Acids Res
Date Published2012 Jul
KeywordsAdenosine Triphosphatases, ATPases Associated with Diverse Cellular Activities, Cell Cycle Proteins, Cell Line, Tumor, DNA, Mitochondrial, HEK293 Cells, Humans, Membrane Proteins, Mitochondria, Mitochondrial Proteins, Nuclear Proteins, Nucleoproteins, Protein Biosynthesis, Repressor Proteins, Ribosomes, RNA, RNA, Messenger, RNA, Mitochondrial

Mitochondrial ribosomes and translation factors co-purify with mitochondrial nucleoids of human cells, based on affinity protein purification of tagged mitochondrial DNA binding proteins. Among the most frequently identified proteins were ATAD3 and prohibitin, which have been identified previously as nucleoid components, using a variety of methods. Both proteins are demonstrated to be required for mitochondrial protein synthesis in human cultured cells, and the major binding partner of ATAD3 is the mitochondrial ribosome. Altered ATAD3 expression also perturbs mtDNA maintenance and replication. These findings suggest an intimate association between nucleoids and the machinery of protein synthesis in mitochondria. ATAD3 and prohibitin are tightly associated with the mitochondrial membranes and so we propose that they support nucleic acid complexes at the inner membrane of the mitochondrion.

Alternate JournalNucleic Acids Res.
Citation Key10.1093/nar/gks266
PubMed ID22453275
PubMed Central IDPMC3401451
Grant ListMC_U105663148 / / Medical Research Council / United Kingdom
MC_U105663140 / / Medical Research Council / United Kingdom
/ / Intramural NIH HHS / United States
BB/F012802/1 / / Biotechnology and Biological Sciences Research Council / United Kingdom
MC_U105697134 / / Medical Research Council / United Kingdom