Online analysis and process control in recombinant protein production (review).

TitleOnline analysis and process control in recombinant protein production (review).
Publication TypeJournal Article
Year of Publication2012
AuthorsPalmer, SM, Kunji, ERS
JournalMethods Mol Biol
Volume866
Pagination129-55
Date Published2012
ISSN1940-6029
KeywordsBiosensing Techniques, Biotechnology, Hydrogen-Ion Concentration, Recombinant Proteins, Temperature
Abstract

Online analysis and control is essential for efficient and reproducible bioprocesses. A key factor in real-time control is the ability to measure critical variables rapidly. Online in situ measurements are the preferred option and minimize the potential loss of sterility. The challenge is to provide sensors with a good lifespan that withstand harsh bioprocess conditions, remain stable for the duration of a process without the need for recalibration, and offer a suitable working range. In recent decades, many new techniques that promise to extend the possibilities of analysis and control, not only by providing new parameters for analysis, but also through the improvement of accepted, well practiced, measurements have arisen.

DOI10.1007/978-1-61779-770-5_12
Alternate JournalMethods Mol. Biol.
Citation Key10.1007/978-1-61779-770-5_12
PubMed ID22454120
Grant ListMC_U105663139 / / Medical Research Council / United Kingdom