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MRC Mitochondrial Biology Unit


Bosco Jose

Preferred first name: Dr Bosco









Dr Bosco Jose studied Doctor of Veterinary Medicine (BVSc & AH) at the prestigious Madras Veterinary College, Chennai, India. After that, Dr Bosco completed his master’s and doctoral program in Veterinary Physiology at the Indian Council of Agricultural Research- Indian Veterinary Research Institute, India. He studied the effect of heat stress on two indigenous breeds of cattle, which is more relevant in this climate change scenario. Following that, Dr. Bosco worked on the generation of gene-edited goat embryos using CRISPR/Cas gene editing tool in his doctoral program under a supervisor, Dr Mihir Sarkar, director, ICAR-NRC-Yak, India. Afterward, He joined as a postdoctoral research fellow at ICAR-NDRI, India for a short period to generate myostatin (MSTN) KO cell lines. He is now working at Prof Edmund Kunji’s lab at the esteemed MRC-MBU, University of Cambridge to generate citrin (aspartate glutamate carrier)/aralar KO HepG2 cell lines.

Research Interests

Dr Bosco Jose is focused to produce knock-out or knock-in mammalian cell lines to generate models for various diseases. Now, he is working on HeLa and HepG2 cell lines to generate citrin/ aralar knock-out or knock-in to understand the citrin deficiency in detail.  He prefers the ribonucleoprotein (RNP) format over others (plasmid & RNA) to target his gene of interest in his CRISPR experiments.

Selected Publications

Jose Bosco, Meeti Punetha, Manoj Kumar Tripathi, Shivani Khanna, Vijay Yadav, Amit Kumar Singh, Brijesh Kumar, Kiranjeet Singh, Vikrant Singh Chouhan, and Mihir Sarkar (2023)
CRISPR/Cas mediated disruption of BMPR-1B gene and introduction of FecB mutation into the Caprine embryos using Easi-CRISPR strategy.
Theriogenology 211: 125-133

Sai Kumar, Meeti Punetha, Bosco Jose, Jaya Bharati, Shivani Khanna, Arvind Sonwane, Jonathan A. Green, Kristin Whitworth, Mihir Sarkar (2020)
Modulation of granulosa cell function via CRISPR‑Cas fuelled editing of BMPR‑IB gene in goats (Capra hircus).
Sci Rep 10: 20446

Meeti Punetha, Sai Kumar, Avishek Paul, Bosco Jose, Jaya Bharati, Arvind Sonwane, Jonathan A. Green, Kristin Whitworth, Mihir Sarkar (2021)
Deciphering the functional role of EGR1in Prostaglandin F2 alpha induced luteal regression applying CRISPR in corpus luteum of buffalo.
Biol Res 54, 9